Detection of matrix metalloproteinase 9 (MMP-9), neutrophil gelatinase associated lipocalin (NGAL) and their complex in serum and urine of dogs with mammary tumors

• Main Authors: Yu-Han Huang, 黃俞涵
• Other Authors: 張仕杰
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/89812512158392317530

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 105 === Matrix metalloprotease-9 (MMP-9), which accelerates breakdown of extracellular matrix associated with tumor invasion and metastasis, can form a high molecular weight complex with neutrophil gelatinase-associated lipocalin (NGAL). The aim of this study was to investigate the expression of MMP-9, NGAL and their complex in dogs with mammary gland tumor. In total, 70 serum and 57 urine samples from 70 dogs were included in this study. Both NGAL and MMP-9 in serum and urine were detected by western blotting. The 130 kDa molecular weight protein was assigned as MMP-9/NGAL complex. There was non-significant difference of MMP-9/NGAL complex present in serum between dogs with mammary gland tumor and tumor-free dogs. In urine samples, MMP-9/NGAL complex was detected of almost 80% of dogs with either malignant or benign mammary gland tumor, but not in urine from tumor-free dogs. The 92 kDa monomer form of MMP-9 and the 25 kDa monomer, 46 kDa dimer and 70 kDa timer form of NGAL were detected in a few urinary samples. The results of expressions of MMP-9, NGAL and their complex in urine and serum were not associated with the type of mammary gland tumor, staging, grading and other clinical characteristics, whereas significant correlation were observed between expression of urinary MMP-9/NGAL complex and mammary gland tumors with infiltration of inflammatory cells according to histopathology. Clinically, these data revealed that MMP-9/NGAL complex was not expressed in urine of tumor-free dog but detection of MMP-9, NGAL and their complex in urine may not predict the progression and prognosis of canine mammary gland tumors.