| Summary: | 碩士 === 國立中興大學 === 生物科技學研究所 === 105 === Tea is a common drink in daily life, drinking tea is generally considered with many health benefits, such as weight loss and reduce deposits of fat. Inhibition of lipase activity is one of the major treatment of diet-induced obesity recently. By inhibiting fat become fatty acid and monoacylglycerol, it prevent over intake of calorie. Orlistat is a clinical drug for weight loss through the mechanism which inhibit lipase activity. According to previous studies, the pu-erh tea crude extract can inhibit lipase activity. Our lab has discovered that raw pu-erh tea contained large amount of strictinin. Strictinin might be a possible cardiant as lipase inhibitor. In the beginning of this study, lipase activity inhibition was test by various kinds of tea in vitro. Preliminary evidence suggests that crude extract of raw pu-erh tea has stronger inhibitory effect than other kinds of tea. Then, strictinin was further isolated from raw pu-erh tea by Sephadex LH-20 column. In the lipase inhibition assay, Strictinin show strong lipase inhibition effect. Thus, strictinin was further tested through animal model. In olive oil tolerance test, the result indicated that the group with strictinin (130 mg/kg) administration of C57BL6 mice has lower triacylglycerol concertation in blood than the group without strictinin. In a long term experiment, the group having high-fat diet with strictinin (130 mg/kg) also has lower weight gain and less adipocyte buildup. These results indicate that strictinin in raw pu-erh tea inhibit the activity of lipase to reduce the fat uptake in food, also control the body weight. Therefore, it will have therapeutically potential to relieve metabolic syndrome.
|
|---|
