Genomics analyses of Klebsiella pneumoniae K62

• Main Authors: Xin-Ying Huang, 黃歆穎
• Other Authors: Ying-Tsong Chen
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/31654783635720397686

碩士 === 國立中興大學 === 基因體暨生物資訊學研究所 === 105 === Klebsiella pneumoniae is a Gram-negative bacterium belonging to the family Enterobacteriaceae. It is originally considered an opportunistic pathogen that causes hospital-acquired infections. Reports of community-acquired infection caused by virulent K. pneumoniae strains were increasing worldwide. K. pneumoniae can be classified into about 80 K-types based on the capsular polysaccharide (CPS). Among these, K1 and K2 are the dominant K-types in Taiwan and were reported in invasive infections such as pyogenic liver abscesses. Previously we have identified a genotoxic phenotype among the K. pneumoniae clinical isolates in a hospital. Subsequent analysis revealed a K62 clone among the genotoxic K. pneumoniae strains, which is likely a emerging virulent clone widespread in Taiwan. Since current information on the genome of K62 is scarce, we have conduct complete sequencing and analyses on the genome of a K. pneumoniae K62 genotoxic strain HLH157. Annotation and comparative genomics analyses with other K. pneumoniae genomes were carried out to identify virulence genes and the pathogenic genomic island components. Moreover, comparative analyses on the genomic region of the CPS genes were carried out to identify K62-specific sequences. A rapid PCR detection method of K62 were developed and the test on our K. pneumoniae clinical isolates appeared to surpass the current K-typing methods. The PCR detection developed in the present study will build a solid basis for future epidemiological study on K. pneumoniae K62 and enhance the study on this emerging virulence clone.