Therapeutic efficacy and apoptosis signaling of marine secondary metabolites, 14-deoxycrassin from Taiwanese soft coral Lobophytum crassum on prostate cancer cell lines

• Main Authors: Chieh-Ju Lin, 林玠如
• Other Authors: Chi-Chien Lin
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/90512891857415492540

碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 105 === Prostate cancer is one of the most common cancer entities as men get older, with bothersome urinary symptoms. Therefore, novel therapeutic agents with low cytotoxic action against normal cells are urgently needed. In this study, we found that 14-deoxycrassin, the cembrane diterpenoids belong to a large family of natural products having a characteristic 14-membered carbocyclic ring skeletal, purified from the soft coral Lobophytum crassum (Von Marenzeller, 1886) was collected at the coast of southern Taiwan, within 6.25-25 μM clearly decreased cell viability of human PC-3 prostate cancer cells dose-dependently which was revealed by Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. Additionally, treatment of PC-3 cells with 14-deoxycrassin displayed an induction of apoptosis by DNA fragment in treated cells. Western blot analyses further revealed that 14-deoxycrassin induces apoptosis by increasing the levels of Bax, while decreasing the levels Bcl-2. As a result, these data suggested that 14-deoxycrassin may be a promising novel agent for treatment and prevention of prostate cancer.