Investigation of the association of phthalate exposure with metabolic activation of estrogen in Taiwanese pregnant women

• Main Authors: Sheng-Yen Hsu, 許聖言
• Other Authors: Po-Hsiung Lin
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/62418739765151565378

碩士 === 國立中興大學 === 環境工程學系所 === 105 === Both 17β-estradiol-2,3-quinone (E2-2,3-Quinone) and 17β-estradiol-3,4-quinone (E2-3,4-Quinone) are reactive metabolites of estrogen that are thought to be responsible for the estrogen-induced genotoxicity. The aim of this study was to analyze estrogen quinone-derived adducts in hemoglobin (Hb) derived from pregnant women (n=394) and to investigate the associations of these adducts with levels of phthalate metabolites in urine and growth and development of newborns. Results confirmed that levels of estrogen quinone-derived adducts in pregnant women. E2-3,4-Q-2-S-Hb and E2-2,3-Q-4-S-Hb were detected with median levels at 160 (range 108-428), 95 (range 35-251) pmol/g, respectively. Levels of E2-3,4-Q-2-S-Hb correlated significantly with those of E2-2,3-Q-4-S-Hb (correlation coefficient r=0.551, p<0.001). The urinary MiBP (Diisobutyl phthalate) positively correlated with those of E2-3,4-Q-2-S-Hb (r=0.106, p=0.04) and E2-2,3-Q-4-S-Hb plus E2-3,4-Q-2-S-Hb (r=0.113, p=0.028). Further investigation indicated that the height and finger length of newborns negatively correlated with levels of E2-3,4-Q-2-S-Hb and E2-2,3-Q-4-S-Hb (p<0.05). Overall, this evidence suggests that the background levels of these adducts in human hemoglobin associates with environmental exposure to phthalates and that environmental exposure to phthalates may enhance bioactivation of estrogen to quinones and interrupt normal growth and development of newborns.