Characteristics of Pseudomonas guguanensis sp. nov. and its electrochemical activity

• Main Authors: You-Cheng Liu, 劉祐誠
• Other Authors: 楊秋忠
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/68214533177633906550

博士 === 國立中興大學 === 土壤環境科學系所 === 105 === Electricity is the most popular energy type in modern society. Under the condition that the global energy demand continues to raise, the development of renewable energy has demanded immediate attention. Microbial fuel cells are an important part of renewable energy research in recent years. Microorganisms can ultilize various hydrocarbons or other complex substrates in sewage as their carbon sources and produce electricity in the fuel cell system. Therefore, microorganisms have played a role in energy security and environmental protection. In this study, 45 isolates were isolated from hot spring water, shallow sand and paddy field of these 45 isolates, 36% of them were Firmicutes, 61% of them were Proteobacteria and 2% of them were Actinobacteria. The strain G9A isolated from the hot spring water was identified as a novel species named Pseudomonas guguanensis. It’s an aerobic, rod-shaped, Gram-negative bacteria with single flagellum, and has deposited at the Bioresource Collection and Research Center, Taiwan (BCRC 80438T = JCM 18416T). For electrochemical detection, the screen-printed electrode (SPE) was immersed in pH 6.8, 0.1 M PBS buffered bacteria solution, and a 0.4V voltage was given for 600 seconds to carry out the electrode surface modification. Eight strains showed electrochemical reactions. G9A had new reduction and oxidation peak at 0.2V and 0.3V, suggesting that the surface of G9A contains redox materials. In differential pulse voltammetry and square wave voltammetry experiment, the oxidation current signal of G9A was significantly increased from -1.16 A to -15.5 A. Suggesting that Pseudomonas guguanensis G9A has the potential to develop microbial fuel cells. The power generation capacity of Pseudomonas guguanensis G9A was measured in dual-chamber fuel cell system by using 5mM sodium acetate as the carbon source and graphite paper (50 cm2) as the working electrode. Power generation was stable under continuous carbon source supply. The current density ranged from 46.9 to 49.8 mA/m2 and the maximum power was 0.03675 mW (7.35 mW/m2).