Pseudomonas aeruginosa EBL6 production of biosurfactant Screening and specificity analysis

• Main Authors: LIN,JING-HONG, 林景弘
• Other Authors: CHEN, HSI-JIEN
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/73999925071264959620

碩士 === 明志科技大學 === 環境與安全衛生工程系環境工程碩士班 === 105 === In this study, we used the highly effective bacteria for the production of surfactant, which was used in the biological treatment and resourceization of the drain pipe in the oil . The method to carried out was using the oil store in the surrounding life, and collected the sludge as the bacteria source. After isolation and purification, the dominant strains EBL1~6 were screened and analyzed. The results showed that EBL2,3 and 6 had the ability of producing surfactant. The results showed that EBL6 was Pseudomonas aeruginosa. Finally, the optimal amount of EBL6 was determined by single carbon and double carbon sources. The metabolites were extracted for purified, qualitative and quantitative analysis. After that,the EBL1~6 was subjected to CTAB experiment, which could form halo in different degrees. The ability of EBL6 halo was the highest, the minimum surface tension was 37dyn / cm and the ability of emulsified diesel oil was determined. The carbon source was added with MSB 1g / L Gluocse was the best growth. The dry weight of the cells was 1.1 g / L and the growth rate was 0.0393 (h-1). The growth of stain was increased by the addition of Glucose to the 50th day, and the dry weight of the cells was 1.74 g / L. Qualitative analysis of EBL6 metabolites for FTIR analysis of the structure with rhamnolipid hydrophobicity and hydrophilicity. RhaC10C10, RhaC10C10, RhaC10H2 and RhaC10C12 were obtained by gradient elution. Quantitative analysis The total sugar content of rhamnolipid was 204mg / L by scallion-sulfuric acid method. Glucose fermentation was carried out with 1g / L of each MSB. 1.02g / L mice were obtained for each 200mL culture flask Li sugar and fat products. The above results show that the use of oil shop sewer separation of EBL6 has a good product of surfactant characteristics and amount, the future can be added to the fermentation tank to increase production and application in the contaminated site simulation processing for the environment.