Effects of riboflavin photolysis on inactivation of Staphylococcus aureus and degradation of crystal violet

• Main Authors: Hsieh, Zong-Jhe, 謝宗哲
• Other Authors: Liang, Ji-Yuan
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/86775260387318906881

碩士 === 銘傳大學 === 生物科技學系碩士班 === 105 === Riboflavin-5′-phosphate　and riboflavin are light sensitive and required in many cellular processes. Their photochemical reactions produce reactive oxygen species (ROS).This study investigated the effects of blue and violet light illumination with Riboflavin-5′-phosphate on Staphylococcus aureus and MRSA. Violet light was more efficient than blue light in such a photolytic inactivation of S. aureus at same radiant intensity. Crystal violet is a dye and an antibacterial drug. The pollute water include crystal violet would destroy the water ecology. This study investigated the blue light with riboflavin to development of an effective degradation of crystal violet treatment. The riboflavin photochemical treatment with blue light was proved to be able to degrade crystal violet dye by reactive oxygen species generated. This novel photodynamic technique could be a simple and safe practice for environmental decontamination.