Study of pumpkin SOD transgene on the chilling tolerance in Arabidopsis

• Main Authors: Sei, Sin-Ci, 謝欣錡
• Other Authors: Chiang, Chih-Ming
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/69911506618231572913

碩士 === 銘傳大學 === 生物科技學系碩士班 === 105 === We use Agrobacterium tumefaciens GV3101 to transfer DNA to arabidopsis, and selected by antibiotic hygromycin. The efficiency of plasmid CmSOD,AtMnSOD and atmnsod’s transformation in arabidopsis are 0.18%、0.14% and 0.22%. Under chilling stress, We can observed the increased of CmSOD transgenic line plant’s CmSOD gene relative expression level, and the CmSOD5 and CmSOD9 transgenic plant’s CmSOD gene relative expression is higher than no stress more 1500% and 600%.Beside ,no matter under chilling stress or not , all AtMnSOD plant’s AtMnSOD gene relative expression is better than NT .And, under chilling stress AtMnSOD 4 and AtMnSOD 5 plant’s AtMnSOD gene relative expression are the most hightest of all AtMnSOD transgenic’s plant in AtMnSOD transgenic plant , they higher than NT more 4 folds. Under chilling stress, atmnsod 4 and atmnsod 7 ‘s AtMnSOD gene relative expression is decrease, and so as NT. In atmnsod 4 , it decreased 80% AtMnSOD gene relative expression.