Study on Separation of Ginsenoside Rg1 and Ginsenoside Rb1 from Panax Notoginseng.

• Main Authors: HO,TA-CHIH, 何達智
• Other Authors: HUANG,MING-HSIEN
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/3t4tkg

碩士 === 國立高雄應用科技大學 === 化學工程與材料工程系博碩士班 === 105 === Panax notoginseng saponins are effective active ingredients in Panax notoginseng, which have been known to have effect on hemostasis, blood stasis, aid in blood circulation and pain relief. In this study, the extraction process of Panax notoginseng saponins was extracted by RO water, 60% methanol solution, 3% NaHCO3 aqueous solution, 3% citric acid aqueous solution and 3% citric acid with 3% salt solution from Panax notoginseng powder. The extracted solutions were obtained by applied ultrasonic waves, and poured into HP-2MG adsorbent resin columns for adsorption and desorption. The eluted solutions were analyzed by FT-IR, UV-Vis and HPLC. The results showed the highest yield of Panax notoginseng saponins, extracted with 60% methanol solution, was 7.51%. The ginsenoside Rg1 and ginsenoside Rb1 were separated by desorbing with 30% methanol solvent and 60% (pH9)methanol solvent from the HP-2MG column, respectively.