Chemical Constituents of the Fungus Diaporthe arecae Derived from Kandelia obovata

• Main Authors: Yan-Yi Lu, 陸彥伊
• Other Authors: Yuan-Bin Cheng
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/89455207217502973459

碩士 === 高雄醫學大學 === 天然藥物研究所碩士班 === 105 === Mangrove is littoral shrub distributes worldwide in subtropics and tropics. The mangrove ecosystem is a high humidity, high salinity, and hypoxia tolerant ecosystem which supports the existence of many halotolerant and halophilic microorganisms. Mangrove-derived fungi are regarded as abundant sources of bioactive natural products and have aroused great interests of natural product chemists. In our screening of Taiwanese mangrove-derived fungi, 20 species of mangrove-derived fungi were successfully purified and cultured. The EtOAc extracts of all isolated fungi were evaluated for anti-cancer activity. As a result, the EtOAc extract of Diaporthe arecae isolated from Kandelia obovata showed potent cytotoxic activity against A549, HepG2, and MDA-MB-231 human cancer cell lines. Accordingly, a series of bioassay-guided for this fungus was carried out. After repeated chromatography, one new indoleglycerol (1) and 23 diketopiperazines (2−24), were isolated from the culture broth of D. arecae. The structures of all isolates were determined by their mass and NMR (COSY, HMBC, and HSQC) spectroscopic data. In the anti-angiogenic assay, cordysinin A (6) displayed mild inhibited effect by suppressing tube formation with an IC50 value of 15.2 μg/mL.