The Effect of Metal Exposure and Oxidative Damage on Urolithiasis

• Main Authors: LIN, JIA-WEI, 林佳葳
• Other Authors: CHEN, HSIU-LING
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/r52929

碩士 === 弘光科技大學 === 職業安全與防災研究所 === 105 === Urolithiasis is a global urinary system problem, including cultures, ethnic groups are likely to suffer from the disease. Recently, the risk factors of urolithiasis are fatty, hypertension and diabetes, demographic factors, genetic, dietary intake, water intake, geographical location, seasonal and accompany with other diseases. The objectives of this study are to measure the metals concentration in urine stone and blood simultaneously in the urolithiasis patients. Meanwhile, the oxidative stress and oxidative damage are also evaluated in the case and control group for evaluating the associations between metal exposure of urolithiasis patients and oxidative damages. The urolithiasis patients were first selected in hospital in central Taiwan and they were recruited into the case group when giving a positive response for joining the study. This study design was approved by the Ethics Committee of the Kuang-Tien General Hospital (Taichung, Taiwan) and all of participants have signed the consent before the sampling started. The control group was recruited in a general community in central Taiwan. In the beginning of the study, the subjects of the control group was set to match for age and sex of the case group, In the present study have been collected 35 case group, 34 control group in Central Taiwan, The blood, urine and stones are collected for metals, and oxidative damages analysis. e, the highest nickel was 172μg/g, which was zinc> lead> copper> chromium> arsenic> cadmium, and the lowest was mercury. The average concentration of heavy metals in blood, the highest Cu was 0.853μg/L, which was Zn> Ni> Hg> Cr> Pb> Cd, and the lowest was arsenic; the average concentration of heavy metals in the control group, the highest Cu was 0.667μg/L, which was Zn> Pb> Ni> Cr> Cd> Hg, the lowest was As. The MDA (2.63µM) in case group is significant higher than that in control group (1.37µM) (P<0.05); as well as the tail moment (2.43) in case group is higher than that in control group (1.69) (P<0.05). The significantly positive correlation was found in Hg content and MDA (P=0.006) of urine stone, as well as blood Pb concentration and 8-OH-dG in urine (P=0.019), blood As concentration and tail moment (P=0.046). Whereas Zn concentration and 8-OH-dG is in negative correlation (P=0.036). The correlations between metals in urine stone and blood showed that the negative correlation in Cd (P=0.014), where the positive correlation in Cr (P=0.002). The result concluded that Ni and Zn are predominant in urolithiasis patients, but the distribution of blood and urine stone are not consistent, Except for Ni, Zn, the data indicated that not all of metals can be accumulated in urine stone and not all of metals can cause oxidative damage in urolithiasis patients.