Associations of one carbon nutrients status with inflammatory response and methylation of imprinted gene during pregnancy

• Main Authors: SHEN, YU-LI, 沈育俐
• Other Authors: SYU, RWEI-FEN
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/75575457585580685150

碩士 === 輔仁大學 === 營養科學系碩士班 === 105 === Dietary intake of folate and choline appear to be inversely related to epigenetic process in epidemiological studies. Pregnant women one-carbon choline and folate requirements, not only needed to maintain maternal one carbon metabolism, but also need to provide enough of one-carbon nutritional environment for fetal to growth and development. Folate and choline of status in pregnant with parental cancer diseases risks and combined with one carbon metabolism enzyme gene polymorphism mutation (MTHFR C677T) correlation of inflammation and imprinted gene methylation in pregnancy has not been clearly. Aims of the study were to investigate the associations of pregnancy one carbon nutrients status with cancer risk factors, inflammatory response and methylation of imprinted gene. Fifty pregnant women were recruited from multiple clinic centers and NTU hospital to participate through the study. Blood samples from the pregnant women were collected for measurements of plasma one-carbon nutritients and inflammation levels. Lymphocytic DNAs were extracted for methylenetetrahydrofolate reductase (MTHFR) genotyping and for methylation status of imprinted gene IGF2/H19 (insulin-like growth factor-2) and LINE-1 (long interspersed nucleotide elements). Dietary data were collected by FFQ and analyzed for nutrient intakes. The data revealed that deficiency rates of total choline, folate and vitamin B6 intake (< RDA/AI) among the first trimester pregnant women were 52%, 16% and 64%, respectively. Historical parental obesity in relative to normal-weight parents was associated with higher intake levels of folate and total choline, and with significantly lower plasma free choline levels in pregnant offsprings. MTHFR 677CT/TT genotypes in relative to CC wildtype were associated with higher intakes of phosphotidylcholine and total choline, and with lower plasma folate and betaine levels. Inflammation results show that plasma vitamin B12 and betaine concentrations were negatively correlated with CRP levels. Dietary intake of folate and betaine were a significant negative correlation with LINE-1 methylation, but plasma vitamin B12 and betaine concentrations were positively correlated with the methylation of imprinted gene IGF2/H19. Detailed mechanism is not clear, still needs more research to explore the related causes.