Single-stage biohythane production via co-immobilized Fermentative microorganisms

• Main Authors: Ta Doan Thanh, 謝 達 榮
• Other Authors: Chiu-Yue Lin
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/95085405472988993493

碩士 === 逢甲大學 === 綠色能源科技碩士學位學程 === 105 === This work has been designed to develop a biohythane production process via co-immobilized fermentative microorganisms in single-stage system using glucose substrate. For cells immobilization, procedures to create double-layer, multi-core and single-layer beads was developed. Double-layer beads procedure met many difficulties and was unable to produce enough double-layer beads to apply in hythane production. Multi-core beads were tested in a batch reactor, but all dispersed within 2 days. Single-layer beads lasted much longer compare to multi-core beads and were used in examining the influence of many seed inoculum amount, glucose concentration and pH. The results shows that the change in bacteria ratio resulted in the change of activities of acidogenesis and methanogenesis, which leads to the change in hythane composition. The importance of VFAs accumulation is also needed to be noticed. Glucose concentration is not only related to the total biogas production, but also has an essential role in controlling pH in batch reactor and takes part in the increase of acidogenesis activities. It is known that changing the pH to values of giving optimum acidogenesis and methanogenesis could be applied to adjust the hythane composition. The results of this study brought a better understanding of hythane production in single-stage system.