| Summary: | 碩士 === 大葉大學 === 分子生物科技學系碩士班 === 105 === Lung cancer mortality rates are the highest in the world at present disease, cancer deaths occur mainly due to cancer. There are currently many studies using natural medicine cancer metastasis and proliferation. Effect is a Traditional Chinese herb, effects have been developed to inhibit liver cancer, hyperplasia of pancreatic cancer, skin cancer, breast cancer, and promote apoptosis, but in the lung cancer study is unknow.
We used lung cancer cells with high metastasis CL1-5 as a test template, with different concentrations of extracts of Ligusticum chuanxiong Hort., Ferulic acid, Tetramethylpyrazine cell lines and analyze some of the functional analysis, also used gene chips to compare different concentration effect of ferulic acid on lung cancer cell line CL1-5 gene expression after , and then through the DAVID functional annotation tool analysis downstream signal path.Results showed that Ligusticum chuanxiong Hort. extract and the biological activity of ferulic acid and tetramethylpyrazine in inhibition of lung cancer cell Proliferation, Migration, Mobility, Cell cycle, while also promoting Apoptosis. Ferulic acid can promote FAS signaling to achieve apoptosis effect; promote P21 signal CDK4/6, CDK2 was inhibited up to cell cycle inhibition in G0/G1Phase through inhibition of VEGF signaling that angiogenesis was inhibited; dampen TRAF2/5 and metastasis inhibition.
These experiments have confirmed that extracts of Ligusticum chuanxiong Hort. and their biological activity of ferulic acid and tetramethylpyrazine in inhibition of lung cancer cell proliferation and metastasis, can also promote apoptosis.
Key words: lung cancer, Ligusticum chuanxiong Hort., cell cycle, apoptosis, metastasis
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