The Development of an Exosomes Purification and Inspection System

• Main Authors: Yuan-Huang Gu, 古原煌
• Other Authors: Ming Chang
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22105CYCU5489074%22.&searchmode=basic

碩士 === 中原大學 === 機械工程研究所 === 105 === For a long time, cancer is a disease that leads to paramount mortality, if we earlier can detect and give appropriate treatment, the more higher survival rate will be greatly improved. When cancer cells begin to hyperplasia or transfer, exosomes will be abnormally secreted in the body. So, if we can develop a simple inspection to detect the quantity of exosomes, perhaps that we just build an early warning for cancers. In this study, we developed a system for purification, dilution sampling of exosomes, and inspection of the number of nanoparticles. First of all, capturing the proteins inside the serum through Polyethylene glycol (PEG)-coated Fe_3 O_4 nanoparticles, then the particles were attached to the bottom by using magnetic force to obtain the exosomes supernatant, the supernatant was diluted with deionized water, and finally put the diluted measuring fluid into the nanoparticles inspection system, the exosomes was measured by rapid image processing after Rayleigh scattered light produced by laser irradiation of exosomes. In this study, the Exosome Purification and Dilution Sampling System are not only able to automatically complete the purification and dilution sampling within 3 minutes, but also can effectively reduce the human factors. Besides, the inspection system only need to improve the traditional microscope, and use laser and 3D printing technology to create a dark field observation platform, to inspect nanoparticles quickly. It has low-cost and easy to popularize characteristics, have the opportunity to develop into an equipment to help humans early detection of cancer cells.