Electrical Analysis for Blood Type Identification Using ZnO interdigitated electrodes

• Main Authors: Chen-an Wang, 王晨安
• Other Authors: Yaw-Jen Chang
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/94716563402583189629

碩士 === 中原大學 === 機械工程研究所 === 105 === This paper describes the measurement agglutination test of erythrocytes using the interdigitated electrodes (IDE) with the Zinc oxide (ZnO) nanowire (NW). This device was fabricated by photolithography and hydrothermal method to grow the ZnO nanowire on the interdigitated electrodes. Different types of photoresists were utilized to define the location of ZnO NW selectively onto the gap of IDE. In the study, experiments were conducted based on the manual polybrene method and measured using the techniques of electrochemical impedance and electrical measurement variety volume of the erythrocytes in agglutination test sample. We use curve fitting to analysis then contrast two methods who is better. The results show that the electrical measurements are under agglutination and non-agglutination distinguishable and more sensitive in comparison with the electrochemical impedance analyses. The measured value are discriminatiul and proportioned to the volume of agglutination. Agglutination of a trace volume of 0.2ul erythrocytes can be detected by ZnO NW-IDE, while it cannot be observed by naked eyes. To compare both measurement techniques, the measurement data were then analyzed by curve fitting. For non-agglutination, the measured value of the impedance method is close to that of non-agglutination. It is difficult to distinguish which is agglutination or non-agglutination. The electrical measurements are show the better result than the electrochemical impedance to make the identification.