| Summary: | 碩士 === 中原大學 === 生物科技研究所 === 105 === Accumulating evidence showed that oxidative stress causes gene mutations and increases the morbidity of cancer; however, fruits and vegetables may increase cellular antioxidant capacity for cancer chemoprevention in human being. In this study, we evaluated the antioxidant potential of the leaves of Syzygium samarangense (wax apple leaves, WAL), including boil water extract (WAL-WE) and ethanolic extract (WAL-EE). WAL-WE exhibited better activity to induce Nrf2-ARE pathway in HepG2-C8 cells with stably transfected ARE-luciferase plasmid.WAL-WE also inhibited TPA-induced JB6 P+ cell transformation. We also found that WAL-WE can increases mRNA and protein expressions of Nrf2 and Nrf2 downstream antioxidant/detoxification genes such as HO -1, NQO1 and UGT1A, in HepG2-C8 and JB6 P + cells. Furthermore, WAL-WE was fractionated by silica gel column chromatography into 25 fractions according to the results of thin layer chromatography analysis and column chromatography weight distribution of 179 collections. Fraction 3,6 and 15 exgibited the strongest Nrf2-ARE induction activity in HepG2-C8 cells. These results suggested that WAL-WE might be a potential cancer chemopreventive agent.
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