Effects of emodin on EBV-associated tumor

• Main Authors: Huang, Jing-Yu, 黃淨鈺
• Other Authors: Lin, Tsuey-Pin
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/qup37c

碩士 === 嘉南藥理大學 === 保健營養系 === 105 === Epstein-Barr virus (EBV) is a human herpesvirus; that is associated with several human lymphoid malignancies. Our earlier study found that the ethyl acetate fraction F3a from Polygonum cuspidatum root promoted apoptosis of EBV-positive cells due to its ability to inhibit the NF-B and ERK activation and enhance the cleavage of apoptotic-related proteins, caspase 3 and PARP. The main component of the ethyl acetate fraction F3a was emodin, which identified by reverse-phase high performance liquid chromatography. Therefore, the purpose of this study is to investigate whether emodin can inhibit the tumor growth of SCID mice bearing EBV tumor cells and explore the effect for emodin with sodium butyrate to inhibit the growth of EBV-associated tumor cells. In prevention experiment, emodin (8.4 mg/kg bw/2 day and 33.8 mg/kg bw/2 day) were administered to SCID mice bearing EB virus tumor cells for 50 days. Researchults showed the survival rate and anti-oxidative enzyme (SOD, GPX, CAT, GSH, P450) of mice were not affected. The tumor volume and weight did not decrease, but spleen weight was reduced induced by the EBV-positive tumor cells. In the treatment experiment, emodin (8.4 mg/kg bw/2day) was injected directly into the tumor site, which reduced tumor volume and weight. In the cell model, 45 M emodin combined with sodium butyrate (1.5 or 3 mM) showed synergistic inhibition of EBV-positive tumor cell growth and the effect was sustained for up to 72 hours. The combination of emodin with sodium butyrate reduced the transcription of EBNA1, thus led to decrease of EBV DNA replication and inhibited cell proliferation. Meanwhile, the expResearchsion of BNLF1 mRNA expResearchsion was increased, which Researchult in the reduced phosphorylation of IkBa, exhibited intracellular reactive-oxygen species increasing, activation of apoptotic-related proteins, caspase-3 and cleavage of PARP and caused apoptosis. Therefore, emodin or emodin combined with sodium butyrate can be developed as a therapeutic drug in the treatment of EBV-related tumors.