| Summary: | 碩士 === 長庚科技大學 === 健康產業科技研究所 === 105 === Urine organic acid contains water-soluble metabolites and/or metabolites derived from sugar, amino acid, lipid, vitamin, and drug, which could reveal the human physiological condition. Among those urine organic acids, the benzoic acid, hippuric acid, phenylacetic acid, phenylpropionic acid, 4-hydroxybenzoic acid, 4-hydroxyphenyl acetic acid, indoleacetic acid, 3,4-dihydroxyphenyl propionic acid, and 3-Hydroxyphenylpropionic acid were the eligible candidates for the dysbiosis of gut microbiota. The aim of this proposal is to develop and validate a high performance liquid chromatography (HPLC) and liquid chromatography tandem mass spectrometry (LC-MS/MS) bioanalysis method for the nine organic acids in urine. The method for selectivity of urine sample no interference, and lower limit of quantification 10 ~ 40 ppb, r2 > 0.99 showed good linearity, accuracy in 85.8 ~ 109.7%, precision in 1.4 ~ 13.3%. All results were met the requirement of bioanalytical method validation.
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