| Summary: | 碩士 === 國立中正大學 === 化學暨生物化學研究所 === 105 === ABSTRACT
Non-specific adsorption of proteins is a key concern in biosensing when biomaterial surfaces come in contact with biological environments. Non-specific adsorption often affects the accuracy and precision of a biosensor. Thus, resistance of non-specific adsorptions to surfaces is an important consideration.
In order to effectively prevent the non-specific protein adsorption for biosensors, development of antifouling self-assembled monolayers is a challenge in particular. Previously, Shaoyi Jiang and co-workers have shown that zwitterionic carboxybetaine ligands have good antifouling property and has an additional advantage of using the carboxyl group for bioconjugation. But due to its properties as quaternary ammonium, the stability of zwitterionic carboxybetaine becomes another challenge as it can undergo Hofmann Elimination reaction.
In this work, we are proposing new derivatives of zwitterionic carboxybetaines with the presence of methylene group in the two carbon spacers called α–methyl carboxybetaine and β–methyl carboxybetaine to make it less chance in suffering Hofmann Elimination. The α–methyl carboxybetaine shows a better resistance against Hofmaan elimination reaction thus provides a better performance over other two carboxybetaine series to resist non-specific protein adsorption. The value of resistance to non-specific adsorption before treatment with base is recorded as 0.002 against 10-5 g/mL BSA and 0.0007 against 10-6 g/mL BSA. Moreover, after treatment with base, the resistance against 10-5 g/mL BSA remained the same as 0.002.
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