具有光裂解性之高分子微胞應用於藥物傳輸系統之研究

• Main Authors: HUANG, CHU-CHIN, 黃筑璟
• Other Authors: CHEN, CHING-YI
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/72329169192704289042

碩士 === 國立中正大學 === 化學工程研究所 === 105 === In recent years, self-assembled polymeric micelles formed by amphiphilic copolymers have emerged as promising nanocarriers for anticancer drugs. Among various polymeric micelles, photo-responsive polymeric micelles have received increasing attention in both academic and industrial fields due to their photo-sensitive nature and unique structures. In this study, a series of new amphiphilic triblock copolymers, poly((1-pyrene) methyl methacrylate)-b-poly(ε-caprolactone)-b-poly(2-(methacryloyloxy)ethyl phosphorylcholine) (PPy-b-PCL-b-PMPC, PyM), were synthesized through ring opening polymerization, atom transfer radical polymerization and click reaction. The hydrophobic segments were composed of photodegradable poly(1-pyrene) methyl methacrylate (PPy) and biodegradable polycaprolactone (PCL), and hydrophilic segment was composed of poly(2-(methacryloyloxy) ethyl phosphorylcholine) (PMPC) with zwitterionic characteristic and good biocompatibility. We confirmed the structure and molecular weight of the copolymers by using NMR, GPC, MALDI-TOF MS and FT-IR. The average sizes of PyM1 and PyM2 micelles were 202 ± 18 nm and 275 ± 10 nm measured by DLS, respectively, which corresponded with those measured by TEM images. For the photo-responsive study, the cleavage of pyrene moiety was confirmed by the decrease of the excimer emission and appearance of monomer emission under UV irradiation. Furthermore, PyM2 micelles were utilized to encapsulate doxorubicin, showing encapsulation efficiency and loading content were 63.02 ± 11.48% and 8.08 ± 1.03%, respectively. For drug release study, PyM2-DOX micelles under UV irradiation showed faster release rate than that of non-irradiation micelles around 37℃ at pH 7.4. These results demonstrated PyM2-DOX micelles with photo-responsive property have the potential for applications in controlled drug release. In the future, in vitro cytotoxicity of drug loaded PPy-b-PCL-b-PMPC micelles will be investigated for their potential applications as photocleavable nanocarriers.