Cyto-toxicological evaluation of trinitrotoluene and its potential metabolites by Citrobacter sp. YC4

• Main Authors: Cheng Ma, 馬正
• Other Authors: Chih-Ching Chien
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/95046159371480345332

碩士 === 元智大學 === 生物科技與工程研究所 === 104 === The heavy pollution of the soil and groundwater was accompanied by the development in economy in Taiwan due to the rapid development of the industry and commerce during the past decades. Nitroaromatic compounds, nitrobenzene, nitrotoluene or nitrophenol for example, were extensively used in industry and might release to soil and ground and cause the pollution. These compounds may be carcinogenic and mutagenic and therefore pose great threaten to human health. In this study, we evaluate the cytotoxicity of trinitrotoluene (TNT) using WST-1-based cell cytotoxicity assay and comet assay. Solution of biodegradation of TNT by Citrobacter sp. YC4 (a bacterium isolated from contaminated soil that able to utilize TNT as sole nitrogen source) at different time intervals were also examined for their cytotoxicity effects. Meanwhile, nitroaromatic compounds including 2,4-Dinitrotoluene (2,4-DNT), 2,6-Dinitrotoluene (2,6-DNT) and 4-Dinitrotoluene (4-NT) were tested for their cytotoxicity as well. The results suggested that even TNT was not complete degraded to CO2 and H2O, the toxicity could still be greatly reduced.