Effect of Culture Medium in Production of Glucosamine Using Aspergillus sydowii BCRC 31742

• Main Authors: Jian-Hao Chen, 陳建豪
• Other Authors: Ho-Shing Wu
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/16060765871361834437

碩士 === 元智大學 === 化學工程與材料科學學系 === 104 === Glucosamine is an amino monosaccharide. It is an important part of mucopolysaccharides and chitin. Glucosamine is a human joint lubricant and it can repair and rebuild damaged cartilage and relieve osteoarthritis disease. Recently, the needs of the microbial production of glucosamine have significantly increased since glucosamine production using chemical extraction and hydrolysis of shellfish derived chitin could not satisfy market needs gradually. This study illustrates the preparation medium of glucosamine, and focus on the control of production costs, using a variety of media culture fermentation ingredients and using shake flasks and five-liter fermentation. Aspergillus sydowii was used to the production of fungal glucosamine, and different low cost carbons sources and nitrogen sources were used to find the best production conditions. In terms of increasing the concentration of glucosamine, and shortening of the fermentation time. When PDB (Potato Dextrose Broth) was used to cultivate. 4th generation fungi, we got a glucosamine of 2.8 g/L, and a biomass of 15.1 g/L. When sugar and soy bean hydrolysate were used to cultivate. 1st generation fungi, we got a glucosamine of 2.03 g/L, and a biomass of 13.9 g/L. When molasses and soy bean hydrolysate were used to cultivate 1st generation fungi, we got a glucosamine of 1.27 g/L, and a biomass of 18.7 g/L. The concentration and productivity of glucosamine obtained were 5.11 g/L, and 42.6 mg/L.h, respectively, and a biomass concentration of 32.1 g/L was obtained when sugar and peptone were used in a 5 L fermenter at 30 oC, for 120 hours. The concentration and productivity of glucosamine obtained were 7.17 g/L, and 74.7 mg/L.h, respectively, and a biomass concentration of 41.7 g/L when molasses and soy bean hydrolysate were used in a 5 L fermenter at 30 oC, for 96 hours. The aeration speed used was 2 vvm (liter of air per liter of medium per minute) and the agitation rate used was 300 rpm(revolutions per minute) for the 5 L fermenter. In summary, molasses plus soy bean hydrolysate is a better medium combination than sugar and peptone. The cost of molasses and soy bean hydrolysate for the production of glucosamine is calculated to be 130 NT/Kg, excluding isolation and purification expensive. For industrial operation, a scale-up procedure such as constant oxygen transfer rate (OTR) or power input (P/V), can be followed to ensure a specified productivity. If isolation and purification cost is not dominating the our all process cost then a continuous fermentation process may be desirable.