| Summary: | 碩士 === 國立陽明大學 === 醫學生物技術暨檢驗學系 === 104 === Understanding the mechanisms of lung cancer metastatic progression was essential to reduce its morbidity and mortality. Analysis of the secretomes from cancer cells may facilitate study of the progression of metastasis, particularly within the phases of migration and invasion. Higher levels of Brain acid soluble protein 1 (BASP1), N-acetylgalactosamine-6-sulfatase (GALNS), Oncostatin-M-specific receptor (OSMR) and Progestagen-associated endometrial protein (PAEP) were secreted by CL1-5 cell. Among them, knockdown of BASP1, GALNS and PAEP affected the migration and invasion ability of CL1-5 cells. Protein levels of four candidates in healthy controls and patients were analyzed by ELISA. The average GALNS, OSMR and PAEP levels were significantly higher in lung cancer patients than the normal control. To investigate the influence of BASP1, GALNS, OSMR and PAEP in lung adenocarcinoma metastasis, label-free quantitative proteomics approach was applied to compare the secretomes of CL1-0, CL1-5, and CL1-5 transfected with lentiviral vector control and shRNA knockdown cells. The proteomics results suggested that knockdown of BASP1, GALNS and PAEP altered the expression of some of the metastasis-related proteins. Next analyzed the expression differences between CL1-0 and CL1-5 cell by RNA sequencing. These findings indicated that numerous genes and transcripts were overexpressed in CL1-5 cells. Taken together, this study suggest that BASP1, GALNS and PAEP affected CL1-5 cell migration and invasion and GALNS, OSMR and PAEP may be used as personalized biomarkers for monitoring non-small cell lung cancer.
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