Development of ROS-Responsive polymeric Micelle in Cytosolic Drug Delivery System.
碩士 === 國立陽明大學 === 生物醫學工程學系 === 104 === Cytosolic drug delivery plays an important role in drug delivery area. The reason is that some drugs are not stable in normal physiological environment, and easily hydrolysis by enzyme during transporting in circulation system. Also, some drugs effect sites are...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2015
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| Online Access: | http://ndltd.ncl.edu.tw/handle/24614287076338385481 |
| Summary: | 碩士 === 國立陽明大學 === 生物醫學工程學系 === 104 === Cytosolic drug delivery plays an important role in drug delivery area. The reason is that some drugs are not stable in normal physiological environment, and easily hydrolysis by enzyme during transporting in circulation system. Also, some drugs effect sites are organelles. Due to what mentioned above, the importance of cytosolic drug delivery would be emphasized.
In this study, we synthesis oxidative-responsive amphiphilic diblock copolymer methoxy-poly(ethylene glycol)-b-poly(diethyl sulfide) (mPEG-b-PDES) and prepare micelle consisting of mPEG-b-PDES and α-tocopheryl succinate (α-TOS) by solvent exchange method. The size and particle distribution index (PDI) of micelles are about 220nm and 0.1 as well as drug loading capacity and efficiency being about 42.2±3.25w.t. % and 32.25±1.87w.t. %. Micelle is stable at simulated physiological environment for at least 42 days. The drug release rate of α-TOS-loaded micelles is nearly double in HCT116 cancer cell line than L929 normal cell line. Cytotoxicity of α-TOS-loaded micelle is strong for HCT116 but not in L929. Micelle escapes from endosome through proton sponge effect, and the escaping period is earlier in HCT116 than L929.
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