Effects on metals and oxidative stress in urine from exposure to environmental tobacco smoke (ETS)

• Main Authors: Yu-Chi Lin, 林侑琪
• Other Authors: Hsien-Wen Kuo
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/04566199938228560930

碩士 === 國立陽明大學 === 環境與職業衛生研究所 === 104 === Background /Objectives: Generally, secondhand smoke (Environmental Tobacco Smoke (ETS), contains a variety of harmful carcinogens, was produced by a cigarette burned. Lack of research was shown the exposure assessment of ETS at home in children and adults. Therefore, the objective the of the study is to investigate the nicotine metabolite levels of ETS among the children and their parents as well as to assess the correlation nicotine metabolites levels with lipid peroxidation damage and heavy metal concentrations. Materials and methods: We completed 318 questionnaires and collected 316 urine specimens from schoolchildren and their parents. Urinary cotinine, 3-OH-Cotinine and lipid peroxidation (MDA) levels were measured using High-performance liquid chromatography (HPLC-UV). Nine heavy metals (As, Cd, Cr, Cu, Hg, Ni, Pb, Se and Zn) in urine were analyzed by inductively coupled plasma-mass spectrometry. Results: Levels of nicotine metabolites among schoolchildren and parents in the exposed group were significantly higher than non-exposed group. Levels of urinary nicotine metabolites correlated some urinary metals in exposed children group and found the urinary heavy metals were significantly correlated with MDA. However, the urinary MDA levels were significantly correlated with urinary levels of heavy metals in the non-exposed parents group. Conclusion: Levels of urinary metabolite of nicotine and MDA concentrations in exposed group of parents and children were significantly higher than in the non-exposed group. Although this study is a cross-sectional study design, there is uncertain regarding causality relationship. It is vital to reduce exposure to secondhand smoke at home to alleviate adverse health effects for people and children.