| Summary: | 碩士 === 國立陽明大學 === 生醫光電研究所 === 104 === Nowadays, biosensors are ideal for the rapid detection in the biological field such as for monitoring the differentiation of stem cells, detection of proteins and their quantification, detection of pathogens, drugs abuse test etc. Among the biosensors, surface plasmon resonance (SPR) biosensors have been considered as a powerful and widely used because of its high sensitivity and real-time detection.
Cocaine, also called as benzoylmethylecgonine or coke. It is a strong stimulant, addictive drug and commonly used for recreation. It inhibits the reuptake of serotonin, norepinephrine, and dopamine, and can easily cross the blood–brain barrier, may lead to the breakdown of the barrier. No matter in U.S.A. or Taiwan, cocaine is one of the most abusive drugs. Is it necessary to build up a system which can rapidly and easily test cocaine or its metabolism, benzoylecgonine (BZE).
According to the literatures, there are many ways can be use in benzoylecgonine detection, including gas chromatography mass spectrometry (GC-MS), liquid chromatography mass spectrometry (LC-MS), and enzyme-linked immunosorbent assay (ELISA). However, we need to go through many processes to prepare the samples for these detection ways.
In our study, we proposed a novel portable surface plasmon resonance system which can easily use, label free and real-time detect the main metabolism of cocaine, benzoylecgonine. The results showed that the resolution of this portable surface plasmon resonance system can reach to 10-5 RIU. We also successfully established a standard curve of benzoylecgonine by using this portable system.
In summary, this potable surface plasmon resonance system provides a real-time detection way for the detection of benzoylecgonine. We expect that this study can not only detect benzoylecgonine but also the metabolisms of other drugs.
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