Development of Renal Function Evaluation by HPLC with Copper Based ECD

• Main Authors: Yu-Chun Lin, 林鈺淳
• Other Authors: Meng-Shan Lin
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/60045627128486640478

碩士 === 淡江大學 === 化學學系碩士班 === 104 === 　　　Both of uric acid(UA) and creatinine(Cr) are important indicator of renal function. For the past years, food additives, industry heavy metal pollution and drug abuse make a severe affect for human health and the population of renal failure is getting higher. For these reason, how to calculate the estimated glomerular filtration rate(eGFR) is always on critical. In this scheme, it developes a determination of HPLC-Cu-ECD to detect UA and Cr simultaneous in human serum and urine and estimate eGFR further. Some reports presented UA and Cr can be chelated with Cu(I) and Cu(II). In this determination, it supplied an oxdation voltage -0.1V(vs. Ag/AgCl) to create the rigid Cu(II) layer on electrode surface. A 50mM phosphate - 75mM acetate buffer solution was used to be a mobile phase at pH7.4 for flow rate 1.0ml/min. UA or Cr will chelate with Cu(II) of the electrode surface then desorption. A oxidative current will be generated when the copper electrode surface formation rearrangement. The detection limit of the determination are presented 0.11 and 0.50μM for UA and Cr which was calculated from 12 times blank injections. The linear range of UA is form 2.5-500μM and Cr is from 10-500μM. The linear range of UA and Cr are over the human serum normal range of UA and Cr. The analytical sensitivity of UA and Cr are 168.06nA/mM and 11.74nA/mM respectively. Both of UA and Cr can be separated other impurities in this scheme that proved it is useful on clinical determination of renal function.