Summary: | 碩士 === 國立臺北科技大學 === 光電工程系研究所 === 104 === The research on D-lactate is allied to the common diseases like diabetes mellitus, hypert-ension, etc, and the principle concentration-detection of D-lactate is a column-switching high-performance liquid chromatography(HPLC). Our group explored the film-based D-lactate flu-orescence biosensor detecting the fluorescence of NADH produced from D-lactate and NAD+ via D-LDH. Herein, a quantum dots(QDs), a colloidal silica, and a D-LDH are coated in the V-groove substrate made on polydimethylsiloxane(PDMS). After the UV light pierces through the D-LDH layer and colloidal silica layer to excite QDs, the concentration of D-lactate is de-duced by the QDs’ fluorescence-intensity. The limit of detection(LOD) of D-lactate biosensor can be reduced by QDs due to the fact that the fluorescence-intensity of quantum dots is high-er than that of NADH. Moreover, these new structure accord with the conditions of the biose-nsor because the generation of NADH is the same as the above. The new system receives a better LOD(6.91μM) compared with the LOD without QDs(8.01μM). Furthermore, we also discuss the effect of QDs at low temperature: Due to the relation to the temperature and the diameter of QDs, the increase of QDs’ fluorescence-intensity at low temperature results in much better LOD(6.36μM). Finally, the experiment shows that these new system accords with the conditions of biosensor: a perfect linear degree(R2=0.9902), an accuracy(4.78-14.82%), a precision(94.4-108.6%), and the error of rat-blood between 9.28-13.97% based on HPLC. In summary, the new design succeeds at decrease in LOD.
|