Research and Testing Protocol Establishment of Tribological Properties between Cartilage-Cartilage Articulation

• Main Authors: Yueh-Yu Tsai, 蔡岳宇
• Other Authors: Hsu-Wei Fang
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/262rm3

碩士 === 國立臺北科技大學 === 化學工程研究所 === 104 === If a tissue engineered cartilage cannot withstand the external stress, it will change the cell viability and the balance between extracellular matrix synthesis and degradation. This will lead to the degeneration of cartilage tissue. However, it is still unclear what the effects of biomechanical variation are when caused by tribology on tissue matrix and cell type. Evaluating the friction and lubricating properties of tissue engineering becomes a topic. This study developed an in vitro testing platform for detecting friction and lubricating properties of tissue engineered cartilage by using pig knee articular cartilage, and discussed the effect of surface geometry, different velocity and lubricants on friction properties of cartilage. In this study, we found the surface geometry was not a main factor to affect friction coefficient when testing coefficient in two seconds after the geometry of cartilage was classified by 3D scanning. I next compared the friction when using PBS or BCS as the lubricant, and observe the friction coefficient of BCS group was slightly higher than PBS group. When testing effect of different velocity on friction coefficient, I observed the friction coefficient of velocity of 3 mm/s was smaller than velocity of 1 mm/s. In the future we plan to use this platform for measuring the friction and lubricating properties of knee articular cartilage, and obtaining data from goat, bovine and rabbit species will be assessed and verified. In addition, we will measure the friction and lubricating properties of tissue engineered cartilages prepared by various biomaterials combined with different culture environments. This will provide a useful database for references and benefit for developing the functional tissue engineered cartilage with excellent friction and lubricating properties. Moreover, we will investigate the changes in biomechanical and lubricative behaviors of the tissue under the stress during tribological process. These results will provide valuable references for functional tissue engineered cartilage.