The Modulation of Chitosan Oligosaccharides on High-fat and High-fructose Corn Syrup Induced Hepatic Manifestations of the Metabolic Syndrome in Murine Model

• Main Authors: Chia-Hao Hsu, 許家豪
• Other Authors: Chang-Chi Hsieh
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/88042727767149751958

碩士 === 東海大學 === 畜產與生物科技學系 === 104 === In recent years, changes in Taiwanese dietary may cause hepatic manifestations of the metabolic syndrome (HMMS). It is associated with the increased fat and fructose consumption observed in the Western diet. Non-alcoholic fatty liver disease (NAFLD) accounts for the majority of chronic liver diseases. It shows high prevalence in almost one third of adults and most obese individuals in those countries. In this study, we want to evaluate of chitosan oligosaccharides (COS) against the onset of HMMs induced by 60% high-fat diet and 30% high-fructose corn syrup in drinking water (HFFD). Mice were divided into five groups, the naive group, the HFFD control group and HFFD with oral chitosan oligosaccharides (150, 300, 600 mg/kg) group, respectively. Serum aspartate transaminase (sAST), triglyceride (TG), total cholesterol (TC) determination in serum or liver and oral glucose tolerance test (OGTT), cytokines in liver, and lipid accumulation in liver tissue sections were determinate by Oil-Red O staining, H&E staining and immunohistochemistry staining for 4-hydroxynonenal (4-HNE), Toll-like receptor (TLR-4) and thymic stromal lymphopoietin (TSLP). Our result indicated COS could reduce mice weight, lipid weight, sAST, sTG and hepatic TG. Furthermore, COS could regulate hepatic interleukin-1β (IL-1β), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), transforming growth factor β1 (TGF-β1), thymic stromal lymphopoietin (TSLP), interferon γ (IFN-γ) and interleukin 4 (IL-4). In addition, the histology stained by Oil-Red O staining, H&E staining and immunohistochemistry staining for 4-hydroxynonenal (4-HNE), Toll-like receptor (TLR-4) and thymic stromal lymphopoietin (TSLP) revealed COS could decreased lipid accumulation and oxidative stress. According to these results, it is suggested that COS could improve lipid accumulation and inflammatory in HMMS.