| Summary: | 碩士 === 東海大學 === 食品科學系 === 104 === Breast cancer is the most prevalent female tumor which causes 460,000 annual deaths. Most of the fatality does not come from breast cancer in its primary site, but rather from the metastasis malignancy. Bone is the major metastasis site of breast cancer,and bone metastasis, which arises mainly by osteoclasts,accounts for over 30% among all secondary tumor cases of breast cancer. Antrodiacinnamomea and Hericiumerinaceusare rich sources of several classes of bioactive compounds. NB-AB, an antroquinonol derivative, was purified from Antrodiacinnamomea,andNB-EA, an erinacine purified from Hericiumerinaceus,both possess anticancer activities and were employed in this study. NB-EA and NB-AB significantly inhibited the differentiation of Raw 264.7 to osteoclast, possibly through MAPK pathway, either in normal differentiation media or in medium supplemented with conditioned media from the culture media of breast cancer cells. Furthermore,NB-EA and NB-ABalso suppressed matrix metalloproteinase 9 (MMP 9) activity and the migration of MDA-MB-231 cells, which might be related to the inactivation of Erk and JNK.In this study, we have demonstrated the anti-metastatic potential and underlying mechanisms of NB-EA and NB-AB in an in vitro cell model.
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