Anti-Inflammation Effects of Tamoxifen in a Traumatic Brain Injury Male Rat Model

• Main Authors: Hu, Chiao-Ya, 胡喬雅
• Other Authors: Kuo, Jinn-Rung
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/65841958845797824040

碩士 === 南臺科技大學 === 生物科技系 === 104 === Purpose: The aim of Anti-inflammation effects of Tamoxifen in a traumatic brain injury male rat model. Material and methods: Anesthetised male Sprague-Dawley rats were divided into four groups as sham operated controls, sham operated controls given tamoxifen (1mg/kg/per day) for 3 days, immediately after Traumatic brain injury (TBI) given vehicle solution for 3 days and immediately after TBI given tamoxifen (1mg/kg/per day) for 3 days. The investigated was evaluate by body weight, Inclined plane, Proprioception. Brain infarction volume were evaluated by 2,3,5-Triphenyltetrazolium chloride (TTC) stain. The total estrogen receptor alpha (ERalpha expression in cortex was also investigated by western blotting. Neuronal loss and apoptosis expression in neuronal cells, Activated Microglia and Astrocyte expression of tumor necrosis factor alpha (TNF-alpha in cortex were evaluated by immunofluorescence stain methods. Results: Compared to those of the sham-operated controls, the TBI-induced behavior were significantly attenuated by tamoxifen therapy on day 4 after TBI. Using immunofluorescence staining, the TBI- induced neuronal loss, apoptosis ,activated Microglia and Astrocyte expression TNF-alpha and ERalpha in cortex are significantly reduced by tamoxifen therapy. Conclusion: Our results suggest that TBI after given tamoxifen (1mg/kg/per day) for 3 days. Tamoxifen may ameliorate TBI-induced behavior in rats by decrease in microglia and astrocyte activation expression TNF-alpha, decrease in neuronal apoptosis, and may thus represent one mechanism by which behavior recovery occurred.