The Determination of Bile Acids And Glycol-Derivatives in Biological Samples With LC-MS/MS.

• Main Authors: CHIU, WEI-JEN, 邱唯禎
• Other Authors: TSAI, SUH-JEN JANE
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/48677052683199350216

碩士 === 靜宜大學 === 應用化學系 === 104 === Cholesterol is the major bile acid metabolite. Therefor bile acid concentration abnormality will lead to different types of disease in the human body. For example primary biliary cirrhosis, gallstones, breast cancer, colorectal cancer, etc. Bile acid is also used to treat diseases. Chenodexycholic acid and ursodeoxycholic acid is used to replace the patient surgical treatment of gallstones. In our study, we used liquid chromatography tandem mass spectrometer (LC-MS) to detect trace amounts of bile acids including cholic acid (CA), deoxycholic acid (DCA), lithcholic acid (LCA), ursodeoxycholic acid (UDCA), chenodexycholic acid (CDCA), hyodeoxycholic acid (HDCA) and their glycine-conjugated form in livers. We combined liquid-liquid extraction, solid phase extraction to save solvent and matrix interferences and multiple reaction monitoring mode (MRM) in mass spectrometer to detect product ion signal cleaved from precursor ions can successful qualitative and quantitative bile acids content in biological samples. Limit of detection 0.53-5.53 ng/mL (S/N=3). Recoveries of bile acids were more than 88% for real samples. In this work, the extraction method is simple and small amount of organic solvents can quickly detect bile acids in biological samples.