Identification of bio-active components from seed coat of black soybean and study of their protection effects on liver injury

• Main Authors: Jhan, Jyun-Kai, 詹鈞凱
• Other Authors: Chung, Yun-Chin
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/86268418263312030417

博士 === 靜宜大學 === 食品營養學系 === 104 === Seed coat of black soybean contain highly amounts of total phenols, anthocyanins and other bio-active compounds, these plant compounds could help to regulation of immunity, prevention of cardiovascular disease, reduction of hyperlipidemia and protection of liver injury on health. There are two parts in this study, the first part was to evaluate the optimal extract solvent and identify of monomeric anthocyanins of black soybean (Glycine max (L) Merr. Tainan No.3), and to investigate the in vitro antioxidation and anti-inflammatory activity. The results showed that contents of total phenolic content, total flavonoids content and total monomeric anthocyaninsseven in seven extracts were 182.13-378.74 (mg gallic acid equivalents/g extract), 4.42-101.37 (mg rutin equivalents/g extract) and 2.15-26.57 (mg cyanidin-3-O-glucoside/g extract), the values of 80% acetone extract (80AE) were the highest. The identification results of LC-MS/MS showed the mainly three anthocyanins in Tainan NO.3 black soybean were cyanidin-3-O-glucoside, peonidin-3-O-glucoside and delphinidin-3-O-glucoside. 80AE not only showed good scavenging ability and inhibition on 1,1-diphenyl-2-picrylhydrazyl (DPPH) (EC50 value was 0.080±0.000 mg/ml), linolenic acid lipid peroxidation (more than 83% was inhibited to day 8) and liposome peroxidation (IC50 value were 0.35 mg/ml), but also get the highest biological activities score during seven extracts. The second part was investigated the protection effects of extracts from seed coat of black soybean (80AE) on CCl4-induced acute and chronic liver injury. The results of acute hepatotoxicity protection experiment showed that the value of CCl4 induced aspartate transaminase (AST) were decreased 46.00% and 52.55% and the value of alanine transaminase (ALT) were decreased 29.51% and 45.38% by pretreated with 50 mg/kg BW or 100 mg 80AE/kg BW for 7 days. The contents of malondialdehyde (MDA) in liver could be maintained in 100 mg 80AE group. The results of chronic hepatotoxicity protection experiment was showed that high dose 80AE (25 mg/kg BW) could increase the activity of catalase (CAT) and glutathione reductase (GSH-Rd) in chronic liver injury rats as similar as silymarin group. 80AE (25 mg/kg BW) also showed inhibition effect on the contents of MDA and the ratio of GSH/GSSG (P＜0.05).