| Summary: | 碩士 === 靜宜大學 === 化粧品科學系 === 104 === In this study, we focus on the effects of commonly used hair dyes , such as Basic red 51, Basic red 76, Basic blue 99 and Hematoxylin on melanogenesis in B16-F10 cells. Firstly, we use MTT assay to confirm the non-cytotoxic concentrations of these agents. Subsequently, these agents were used to test the effects on melanin production, tyrosinase activity and the expressions of microphthalmia-associated transcription factor (MITF) and tyrosinase.The results showed that: Under non-cytotoxic concentrations, Basic red 51, Basic red 76, Basic blue 99 generate no significant role for B16-F10. However Hematoxylin exhibits significantly increased melanin production. Our results indicated that Hematoxylin has abilities to increase the melanin productions of 14.7 %. Hematoxylin has ability to increase the tyrosinase activity 15.2 %. Hematoxylin has ability to increase tyrosinase activity approximately 1.29 fold of control in tyrosinase zymography and also protein levels of MITF and tyrosinase. Therefore, Hematoxylin can evidently increase the production of melanin in B16-F10 Cells.
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