Chemical Constituents From The Stem of Eurya loquaiana

• Main Authors: LO,CHIEH-CHIH, 駱杰志
• Other Authors: YU,HSI-JUNG
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/61863083932000667038

碩士 === 中國文化大學 === 化學系應用化學碩士班 === 104 === Eurya loquaiana belongs to Theaceae family Eurya genus, which grows in the low altitudinal to the high altitudinal mountainous areas. E. loquaiana are distributed in Anhui, Jiangxi, Hubei, Hunan, and Taiwan area. In this thesis, the chemical components of E. loquaiana were studied. The fresh E. loquaiana were collected in New Taipei City area in 2014. The stems of E. loquaiana were cut in small species and powdered and then extracted with MeOH under reflux in four times. The combined MeOH extracts were filtered and concentrated. The residues were then partitioned between EtOAc and H2O. The EtOAc layer was concentrated and repeated chromatographyed on silica gel to yield 5 compounds including -sitosterol (1), 1-oxo-3-hydroxyolean-18-ene (2), betulin (3), betulinic acid (4), and sitosterol-3-O--D-glucopyranoside (5). Water layer was column chromatographyed on ion-exchange resin eluting with H2O, 50% MeOH, 75% MeOH, and MeOH, respectively. The MeOH fraction was further chromatographyed on silica gel or Sephadex LH-20 to yield 3 compounds including lupeol (6), betulinaldehyde (7), and 3,3’,4-trimethoxyellagic acid-4’-O-glucopyranoside (8). Water insoluble fraction was purified to obtain apigenin (9). The structures of compound 9 isolated compounds were elucidated on the basis of spectroscopic data analysis.