Analysis of Nutraceutical Activity for The Extracts Prepared by Supercritical Fluid Extraction from Antrodia cinnamomea

• Main Authors: Chen, Liang-Chung, 陳亮中
• Other Authors: Hwang , Tzann-Shun
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/07346350911509736591

碩士 === 中國文化大學 === 生物科技研究所 === 104 === Anrtodia cinnamomea, a medicinal fungus, grows on the inner surface of decayed Cinnamomum kanehirae in Taiwan ,with an annual market of over $100 million (US) in Taiwan. It is used to treat toxicities caused by food, alcohol, and drugs as well as diarrhea, abdominal pain, hypertension, itchy skin, and tumors. Since its discovery in 1989, hepatitis C virus (HCV) has been recognised as a major cause of chronic liver disease worldwide.Approximately 85% of persons infected with HCV develop chronic hepatitis and may lead to hepatocellular carcinoma. Antrodia cinnamomea is also a highly valued folk medicine used for liver cancer, often caused by long tern infection of heptitis C virus.There are two purposes in this study. To find out the best grain medium for growing Antrodia Cinnamomea, and to examing the ability of Antrodia Cinnamomea extracts from supercritical fluid CO2 extraction against HCV NS3/4A protease. The results showed that the best grain medium for Antrodia cinnamomea was oat medium whatever carbohydrate or protein content was fixed. In the inhibitory activity analysis, the SCF CO2 extraction of solid medium culture Antrodia cinnamomea possessed good inhibit activity against HCV NS3/4A protease with IC50 of 1.2916 μg/ml.