Transgenic microorganism for HMF (5-(hydroxymethyl)furfural) degradation and high-value FDCA (2,5-furandicarboxylic acid) production

• Main Authors: Chih-Ting Hsu, 許芷婷
• Other Authors: Shen-Long Tsai
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/12790301309655457048

碩士 === 國立臺灣科技大學 === 化學工程系 === 104 === Due to the indiscriminate use of fossil resources, the need for renewable feedstocks is become increase evident. 2,5-furandicarboxylic acid (FDCA) is a promising bio-based platform chemical. Production of FDCA needs to oxidize the 5-hydroxymethylfurfural（HMF）. When HMF is oxidized, it can become 5-(hydroxymethyl)furan-2-carboxylic acid (HMF acid), which is intermediate. Then HMF acid is oxidized and product the FDCA. FDCA is a green substitute for terephthalate in polyesters, and it is much friendlier to environmental. In this study, using gene recombination to make two different enzyme, which are called HMFH and HMFO. Inserting the gene of HMFH and HMFO into the vector, then transformate the vector which includes gene of HMFH or HMFO into the bacteria Pseudomonas putida s12. When the solvent-tolerant Pseudomonas putida s12 express, the two enzyme HMFH and HMFO can produce in the bacteria. When add the high concentration of HMF (50mM) to the Pseudomonas putida s12 including HMFH or HMFO, the bacteria work as the whole-cell biocatalyst, HMF is oxidized to FDCA. We combined HMFH and HMFO together to find how to make the production of FDCA maximum. In this result, we found that combine HMFH,HMFO and ALDH have best result, at 2 hours, FDCA could be detected around 15mM and HMF be converted to HMF acid ,which concentration of HMF acid is around 34mM. After 4 hours HMF disappeared, all be converted into HMF acid and FDCA; and after 24 hours HMF acid almost disappear, just only detected FDCA.