Effect of the co-culture on the biomass and lipid content using rice bran hydrolysate as nutrition

• Main Authors: Kuan-Hung Chen, 陳冠宏
• Other Authors: Yi-Hsu Ju
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/10520218554963670068

碩士 === 國立臺灣科技大學 === 化學工程系 === 104 === The demand of petroleum fuel is increasing and 80% of primary energy is produced from petroleum fuel. In order to decrease the consumption of fossil fuels, alternative renewable fuel has been a hot topic in recent years. Microbial oil from oleaginous microorganism is considered as a potential candidate for biodiesel production. Agricultural waste reused can reduce the cost of oleaginous microorganism for lipid production. Rice bran is an underused byproduct from rice milling and is produced in large quantity worldwide. Rice bran contains higher polysaccharide which can be considered as nutrient source for microbial fermentation. In this study, the effect of co-culture on biomass and lipid content using rice bran hydrolysate was investigated. The rice bran was subjected to acid hydrolysis with various hydrolysis time, stirring speed and sulfuric acid concentration. The optimal condition of rice bran hydrolysis was using 2 % sulfuric acid at 90°C for 3.5 h with 300 rpm stirring speed. After detoxifying and neutralizing, the rice bran hydrolysate was used for the co-culture of L. starkeyi and C. vulgaris. It was found that co-culture cannot improve biomass and lipid content. The culturing of L. starkeyi can achieve higher biomass and lipid content than co-culture. The highest biomass, lipid content and lipid production obtained were 9.4 g/L, 34.3% and 3.22 g/L, respectively.