Using Bioengineering To Construct Smart Escherichia Coli For Phenol Degradation

• Main Authors: Ya-Zhu Yang, 楊雅筑
• Other Authors: Shen-Long Tsai
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/91043579599946746213

碩士 === 國立臺灣科技大學 === 化學工程系 === 104 === With the development of petrochemical industry, organic compounds are widely used in many cases, like plastic industry, pesticide, and so on. However, these chemical may be released into the air, water and soil affecting ecosystems and human beings alike. Among the organic compounds, Phenol is one of the most widely used in existence and is a basic structural unit for many industries including agricultural chemicals and pesticides. Phenol also can form other stable substituents in water which called phenolic compounds. But phenol is toxic, carcinogenic, and mutagenic in low concentration, it and its organic compounds are seen as the most important environmental pollutants. In the past, human used many ways to remove the phenolic compounds in the environment, like adsorption, extraction and oxidation. However, these methods cannot remove phenolic compounds effectively and may cause other problems. Bioremediation is efficient way to remove pollutants. It is low cost and eco-friendly. For bioremediation, it is significant for bioremediation to develop a new bacterial strain. So, we want to use synthetic biology to construct a smart bacteria which can degrade phenol. It can sense the phenol in the environment and then trace it. Finally it can degrade phenol. First, we constructed a phenol biosensor. Its reporter will be increased by phenol concentration or induction time. Then, we add che-Z gene in biosensor. Therefore, phenol can active transcription of the che-Z gene. The biosensor with motility can move from low concentration to high concentration. Finally, we constructed the smart E. coli which can degrade phenol. The bacteria can totally degrade 1mM phenol in 24 hr. So, the smart E. coli for phenol degradation can totally remove pollutants around factory. It also can be a model for bioremediation.