Preparation of Electrosprayed Coreshell Nanoparticles for Targeting Brain Tumor

• Main Authors: Yi-Hou Lin, 林宜儫
• Other Authors: Ming-hua Ho
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/86704644933705285875

碩士 === 國立臺灣科技大學 === 化學工程系 === 104 === In this study, chitosan-polylactide (PLA) coreshell nanoparticles were fabricated by coaxial electrospraying. The applied voltage, flow rate and working distance were optimized in this research. After conjugated with folic acid and lactoferrin, the diameter of nanoparticles was not significantly changed. The biocompatibility of nanoparticles with different concentrations were investigated by culturing U87MG (glioblastoma) and SVGp12(glial) cells. We found that nanoparticles with low concentration would not result in significant cytotoxicity, no matter there was surface modification on nanoparticles or not. The nanoparticle conjugated folic acid showed a better efficiency in cellular uptake, which was especially significant for glioblastoma cells. Compared with chitosan nanoparticles, core shell nanopaticle would decrease the burst release due to the existence of PLA shell. The curcumin encapsulated in nanoparticles showed better cytotocixity for glioblastoma cells than free curcumin. If we immobilized folic acid onto nanoparticles, the cytotoxic to U87MG was further promoted. However, curcumin loaded in nanoparticles with and without surface modification showed limited cytotoxicity to glial cells. Finally, the results of in vitro BBB permeability indicated that conjugated lactoferrin on nanoparticles surface enhanced the permeability of nanoparticles to cross BBB.