| Summary: | 碩士 === 國立臺灣科技大學 === 醫學工程研究所 === 104 === In previous studies, ultrasound (US) can induce cell activation around the wound to accelerate wound healing. In this study, a new antibacterial US microbubbles (MBs) as a drug delivery vehicle, loaded with epidermal growth factor (EGF) added to the dressing combines with US was provided to accelerate wound healing.
The Lysozyme-shelled MBs (LYMBs) absorbed EGF on this cationic carriers. The zeta potential and size distribution of the EGF loaded LYMBs in suspension were measured by DLS. The in vitro, anti-bactericidal experiment for S.aureus and in vivo experiments were performed. The experimental parameters of anti-bactericidal experiment for S.aureus will be divided into eight groups (n=3): (1) the control group(C), (2) US combines control group(C+U), (3) only Lysozyme solution(L), (4) US combines Lysozyme solution(L+U), (5) only LYMBs(M), (6) US combines LYMBs(M+U), (7) only EGF-LYMBs(EM), (8) US combines EGF-LYMBs(EM+U). The in vivo experimental parameters will be randomly divided into five groups (n=5 animals per group): (1) no treatment [the control group(C)] and US alone(C+U), (2) only dressing(D) and US combines dressing(D+U), (3) LYMBs in dressing(M) and US combines LYMBs in dressing(U+M), (4) EGF solution in dressing(E) and US combines EGF solution in dressing(E+U), (5) EGF-LYMBs in dressing(EM) and US combines EGF-LYMBs in dressing(U+EM).
The cationic LYMBs were absorbed by EGF. The mean diameters of LYMBs and EGF-LYMBs were 2.68 ± 0.21 μm and 3.78 ± 0.18 μm, respectively. The zeta potentials of LYMBs and EGF-LYMBs were 80.33 ± 1.19 mV and 40.33 ± 3.62 mV, respectively. The loading efficiency of EGF on cationic LYMBs was 19.40%. The EGF release kinetics from EGF loaded LYMBs were determined by dynamic dialysis method. After US treatment, the released rate increased 44% at 2 hours. The results of anti-bactericidal experiment for S.aureus showed the best anti-bactericidal effect (97.50%) at a power density of 3 W/cm2 combined with the LYMBs(M+U) higher in than those in groups C+U(18.23%), L(75.12%) and M(96.56%). The results of in vivo experiments showed the best wound healing effect at a power density of 3 W/cm2 combined with the EGF-LYMBs(EM+U) higher in than other groups. The results confirmed that US combined with EGF-LYMBs can enhance wound healing.
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