Developing a Novel Polymeric Drug Carrier for Both Hydrophilic and Hydrophobic Anti-cancer Drugs

• Main Authors: I-Ting Lu, 盧怡廷
• Other Authors: Chen-Yu Kao
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/50404525822332382328

碩士 === 國立臺灣科技大學 === 醫學工程研究所 === 104 === Cisplatin is one of the most potent anticancer agents used in the treatment of various solid tumors including bladder cancer, testicular cancer and ovarian cancer. It was the first FDA-approved platinum compound for cancer treatment in 1978. However, its use is mainly limited due to the drug resistance and considerable side effects. Also, precence drug is practically insoluble in organic solvent and slightly soluble in water makes the drug difficult to encapsulate into polymeric drug delivery system that can maintain optimum concentration for longer period of time. In order to improve the anti-cancer effects, combination therapy of cisplatin with other cancer drugs has been applied as novel therapeutic strategy for several human cancers. In this research, we combined it with magnolol. Magnolol, the major bioactive ingredient of Magnolia officinalis is known to have antioxidant, anti-inflammatory, anti-neurodegenerative, liver protecting and anti-cancer effects. However, the poor solubility and suspension capacity under physiological conditions have hindered its bioavailability and clinical efficacy. Based on this concept, we prepared the magnolol-loaded nanoparticles by using PEI-PLGA and PEI-PCADK, and cisplatin-loaded micro particles by alginate as drug carrier respectively. After preparing ionic complex of both alginate and PEI-PLGA/PCADK particles, we investigated their encapsulation efficiency, drug release, and the antitumor effect of drug carrier by PLGA or PCADK on oral epidermoid carcinoma cells (OECM-1) and cervical cancer (HELA). The result showed that the surface of PLGA and PCADK is successfully modified by PEI, and maintained the particle morphology and size. Mag-PEI-PCADK-Alg-Cis particle and Mag-PEI-PLGA-Alg-Cis particle can load more magnolol and cisplatin respectively. Both of them showed stable release of both magnolol and cisplatin. The inhibitory effect to OECM-1 and HELA of Mag-PEI-PCADK-Alg-Cis particle is not only better than Mag-PEI-PCADK also than Mag-PEI-PLGA-Alg-Cis particle. These findings reveal a potential therapeutic strategy of oral and cervical cancer.