| Summary: | 碩士 === 國立臺灣大學 === 口腔生物科學研究所 === 104 === Pulp capping is applied to a deep tooth cavity for keeping the pulp vitality. The following light cured composite resin is always applied to recovery the original morphology and chewing function. However, it containing toxic monomers may permeate through dentinal tubules to cause pulp damage. The purpose of this research project is to develop nano-calcium encapsulated mesocellular siliceous foams (denoted as nCa-MCFs) as a capping biomaterial that can form biomimetic crystallization in dentinal tubule when nCa-MCFs works with phosphoric acid (denoted as nCa-MCFs-HP). The biomimetic crystal layer would serve as a protective barrier protect pulp tissues from the toxic monomer of dentin bonding agents, and further induces the differentiation of odontoblast.
This study carries out three parts—Part Ι: To investigate the properties of nCa-MCFs-HP material and the formation of biomimetic crystallization in dentin tubule. We would analyze the interaction of tubule crystallization and biomaterials by using scanning electron microscope. The elution of dentin bonding agents and monomers through 0.2 mm dentin disc after nCa-MCFs-HP treatment would analyzed by UV-Vis spectroscopy and HPLC. Part ΙΙ: To evaluate the biocompatibility of nCa-MCFs-HP biomaterials, and to establish the in vitro deep dentin disc model. Part ΙΙΙ: To evaluate the pulp cell mineralization after nCa-MCFs-HP treatment.
The results revealed that the nCa-MCFs-HP can form calcium phosphates crystallization in tubule. The biocompatibility of nCa-MCFs-HP was also confirmed by WST-1 and LDH test, which indicates no significant cytotoxicity, and lower cytotoxicity than the group of dentin bonding agents. The simulated deep cavity treating with nCa-MCFs-HP material can form a biomimetic crystalline barrier which may protect pulp cell from toxicity monomers released from composite resins and dentin bonding agents. ALP staining assay, ALP quantitative assay, and mineralization-related protein – DMP-1, DSPP expression further indicated the nCa-MCFs-HP treated dentin has the great help of the induction of pulp cell mineralization.
Based on the given situation, we concluded that the nCa-MCFs-HP biomaterial has good biocompatibility, and the forming biomimetic crystallization protect layer could reduce the toxic monomers permeation, protect pulp tissues, and promote mineralization as a pulp capping material.
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