| Summary: | 碩士 === 國立臺灣大學 === 醫學工程學研究所 === 104 === Cell therapy is a kind of treatment method that delivers cells which are cultured in vitro into the human body in order to recover missing or damaged cells. In the past, cells are delivered by injection, but this way may make patients painful, have the possibility for patients to get infected, need trained personnel to operate and have the possibility of forming hematoma or bleeding. In this study, we used microneedle array as a carrier for cells delivering. With its small size, the subcutaneous wound is smaller and it will not touch nerves under the skin, so improvement of patients’ comfort can be easily achieved. Besides, using microneedle array will not require trained personnel to assist operating. Finally, it will reduce the risk of infection and hematoma formation. We used polymethyl-methacrylate (PMMA) as the material for fabricating microneedle array due to its adequate mechanical strength, excellent biocompatibility, stability and lower price. We are looking forward to using microneedle array made of PMMA to act as a carrier for cells delivering in order to treat missing or damaged cells in the human body.
In this study, we used polydimethylsiloxane(PDMS) mold for fabricating PMMA microneedle array. Biocompatibility and cell viability were evaluated by MTT assay. Cell adhesion and proliferation on microneedle array were examined by Live/Dead Staining and SEM. Moreover, we used collagen, alginate and acellular skin as a delivered material to observe whether the cells can deliver from microneedle array to another place.
The results show that PMMA microneedle array has good biocompatibility and cells can attach and grow on it. Besides, cells on the microneedle array can successfully deliver to collagen, alginate and acellular skin. Therefore, microneedle array with cell therapy is a new and highly potential treatment method.
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