Crosslinked Electrospun Fibers Change Scaffold Mechanics and Mechanobiology

• Main Authors: Pang-Ching Liu, 劉邦卿
• Other Authors: Pen-Hsiu Chao
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/31040337232249440241

碩士 === 國立臺灣大學 === 醫學工程學研究所 === 104 === Native ligament is formed by aligned wavy collagen fiber. Previous study has shown that cells have better ECM production on biomimetic wavy electrospun fiber scaffold. But only having architecture similarity is insufficient. Collagen fibers are cross-linked in native tissue which contribute to material’s properties. The aim of this study is to generate bonding fiber and investigate the influence of biomimetic bonded electropsun fibers on material’s mechanical properties and cell physiology. Our results show that bonded fiber has better mechanical properties in transverse stretch without changes in fiber scaffold morphology. Confocal image and mRNA expression also shows that cell has no significant change in morphology and phenotype in bonding fiber. And gene expression response of tenascin-C to mechanical stimulation is different in bond group, which means the bonded fiber has different strain transfer in loading. Future studies will change porosity of PLLA fiber and reduce bonded temperature to optimized mechanical properties of the scaffold and investigate cell’s phenotype.