Calreticulin regulates FUT1 expression and beta1 integrin fucosylation in PC3 prostate cancer cells

• Main Authors: Yi-Cheng Weng, 翁義程
• Other Authors: Hsinyu Lee
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/65136801959781499618

碩士 === 國立臺灣大學 === 生命科學系 === 104 === Calreticulin (CRT) is a multifunctional ER chaperon protein and it had been revealed that knockdown of CRT may suppress cell proliferation and migration in J82 bladder cancer cell. We had further demonstrated that CRT enhanced the expression of fucosyltransferase1 (FUT1), which leads to an enhancement of fucosylation of beta1 integrin, and promote cell adhesion in J82 cells. Previous studies had shown that knockdown of CRT suppress cell proliferation and migration in PC3 prostate cancer cell as well. However, the effects by CRT on integrin fucosylation have not been revealed in prostate cancer so far. In this study, therefore, we attempted to investigate whether CRT affect FUT1 expression and integrin fucosylation in PC3 prostate cancer cells as well. Our results showed that FUT1 expression and beta1 integrin fucosylation are suppressed after knockdown of CRT. Futhermore, cell adhesion and integrin activation are down regulated as well. These observations imply that CRT might regulate prostate cancer metastasis through FUT1 and therefore could be a potential target for development of cancer treatments.