Study on the Immunomodulatory Potential of Regulatory T cells Induced by B Cells in Joint Inflammation

• Main Authors: Szu-Ying Chen, 陳思縈
• Other Authors: Bor-Luen Chiang
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/66685848090390520288

博士 === 國立臺灣大學 === 臨床醫學研究所 === 104 === Rheumatoid arthritis (RA) is an autoimmune disease in which dysregulated immune cells primarily target synovial joints. Despite recent advances in the treatment of RA, including the introduction of biologic therapies and employment of combination disease-modifying antirheumatic drug strategies, remission rates remain suboptimal. Previous studies have demonstrated that the adoptive transfer of induced regulatory T cells (iTreg cells) was effective in treating a murine model of collagen-induced arthritis (CIA). The objective of this study was to develop optimal potential iTreg-based therapy for CIA by adoptively transferring LAG3+ Treg-of-B cells. Our results showed that B-cell-induced Treg-of-B cells expressed LAG3 but not Foxp3 (designated LAG3+ Treg-of-B), and secreted IL-4, IL-10, and TGF-β. Furthermore, LAG3+ Treg-of-B cells suppressed the proliferation of CD4+CD25- responder T cells through both LAG3 and IL-10 production. In the murine CIA model, adoptive transfer of LAG3+ Treg-of-B cells alleviated the joint severity as well as local and systemic inflammation. Treatment with LAG3+ Treg-of-B cells also promoted IL-10 production in lymphocytes isolated from the spleen and draining lymph nodes. Moreover, mice receiving LAG3+ Treg-of-B cell treatment showed significantly less pronounced osteolysis in the hind footpads, which correlated with the downregulation of tartrate-resistant acid phosphatase expression. In conclusion, we identified a novel subset of Treg cells for CIA treatment. This insight may facilitate exploring novel regulatory T-cell-based therapies for human autoimmune diseases.